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Non leukocyte innate immunity

Three main themes are envisaged

- Study of MSC differentiation in follicular reticular cells (FRC), pericytes and/or myofibroblasts . FRC are mesenchymatous cells residing in the lymph nodes where they exert a potent regulatory activity on T lymphocytes. A better understanding of their biology and their relationship with MSC would help deciphering how they modulate T cells in situ, keeping in mind that such a control is pivotal for the maintenance of peripheral immune tolerance.

- Investigation of the effect of MSC via the activation of indolamine 2,3, deoxygenase (IDO) upon tumor cell growth. IDO, through its ability to metabolize the essential amino acid L-tryptophan modulates many cell types, including T cells and several tumoral cells. The intensity of IDO censoring is related to cell sensitivity to tryptophan depletion. IDO direct effect is expected to inhibit tumor proliferation. However if a tumor becomes IDO-resistant and is surrounded by IDO+ cells, the depletion of tryptophan induced by IDO will inhibit tumor specific T cells and favor tumor growth. A better understanding of these processes should help the development of new aspects of anti-tumoral therapies.

- Establishment of a protocol generating clinical grade MSC to be infused to patients suffering from severe GVHD. Many clinical trials suggest that MSC systemic infusion may stop steroid resistant GVHD. However the paucity of GMP grade MSC often prejudices the initiation of such treatment. This project consists in the finalization of the production of MSC in white room conditions.

Group's publications

Implication of indolamine 2,3 dioxygenase in the tolerance toward fetuses, tumors, and allografts.
JOURNAL OF LEUKOCYTE BIOLOGY
2013 vol. 93(5) pp. 681-687
DÜRR S, KINDLER V

Autologous bone marrow mononuclear cell transplantation in patients with decompensated alcoholic liver disease: a randomized controlled trial.
PLOS ONE
2013 vol. 8(1) pp. 53719-53719
SPAHR L, CHALANDON Y, TERRAZ S, KINDLER V, RUBBIA-BRANDT L, FROSSARD JL, BREQUET R, LANTHIER N, FARINA A, PASSWEG J, BECKER CD, HADENGUE A

Impact of selection of cord blood units from the United States and swiss registries on the cost of banking operations.
TRANSFUSION MEDICINE AND HEMOTHERAPY
2013 vol. 40(1) pp. 14-20
BART T, BOO M, BALABANOVA S, FISCHER Y, NICOLOSO G, FOEKEN L, OUDSHOORN M, PASSWEG J, TICHELLI A, KINDLER V, KURTZBERG J, PRICE T, REGAN D, SHPALL EJ, SCHWABE R

Low molecular weight dextran sulfate binds to human myoblasts and improves their survival after transplantation in mice.
CELL TRANSPLANTATION
2012 vol. 0 pp. 0-0
LAUMONIER T, PRADIER A, HOFFMEYER P, KINDLER V, MENETREY J

Third-party mesenchymal stromal cell infusion is associated with a decrease in thrombotic microangiopathy symptoms observed post-hematopoietic stem cell transplantation.
PEDIATRIC TRANSPLANTATION
2012 vol. 16(2) pp. 131-136
ANSARI M, STRUNK D, SCHALLMOSER K, DELCO C, ROUGEMONT AL, MOLL S, VILLARD J, GUMY PAUSE F, CHALANDON Y, PARVEX P, PASSWEG J, OZSAHIN H, KINDLER V

Human bone marrow stromal cells and skin fibroblasts inhibit natural killer cell proliferation and cytotoxic activity.
CELL TRANSPLANTATION
2011 vol. 20(5) pp. 681-691
PRADIER A, PASSWEG J, VILLARD J, KINDLER V

EWS-FLI-1 expression triggers a Ewing's sarcoma initiation program in primary human mesenchymal stem cells.
CANCER RESEARCH
2008 vol. 68(7) pp. 2176-2185
RIGGI N, SUVÀ ML, SUVÀ D, CIRONI L, PROVERO P, TERCIER S, JOSEPH JM, STEHLE JC, BAUMER K, KINDLER V, STAMENKOVIC I

In vitro activated human T lymphocytes very efficiently attach to allogenic multipotent mesenchymal stromal cells and transmigrate under them.
JOURNAL OF CELLULAR PHYSIOLOGY
2008 vol. 214(3) pp. 588-594
SUVA D, PASSWEG J., ARNAUDEAU S, HOFFMEYER P, KINDLER V

Human CD34+ CD11b- cord blood stem cells generate in vitro a CD34- CD11b+ subset that is enriched in langerin+ Langerhans dendritic cell precursors.
EXPERIMENTAL HEMATOLOGY
2006 vol. 34(11) pp. 1471-1479
SOULAS C, ARRIGHI JF, SAELAND S, CHAPUIS B, KINDLER V

Non-hematopoietic human bone marrow contains long-lasting, pluripotential mesenchymal stem cells
JOURNAL OF CELLULAR PHYSIOLOGY
2004 vol. 198 pp. 110-118
SUVA D, GARAVAGLIA G, MENETREY J, CHAPUIS B, HOFFMEYER P, BERNHEIM L, KINDLER V

CD34 cord blood cells expressing cutaneous lymphocyte-associated antigen (CLA) are enriched in granulocyte-macrophage progenitors and support an extensive amplification of dendritic cell progenitors
EXPERIMENTAL HEMATOLOGY
2001 vol. 29 pp. 1029-1037
ARRIGHI JF, ZUBLER RH, HAUSER C, IRION O, BROWERS N, CHAPUIS B, KINDLER V

Transduction of CD34+ cells with lentiviral vectors enables the production of large quantities of transgene-expressing immature and mature dentritic cells
THE JOURNAL OF GENE MEDICINE
2001 vol. 3 pp. 311-320
SALMON P, ARRIGHI JF, PIGUET V, CHAPUIS B, ZUBLER RH, TRONO D, KINDLER V

Research's domains




DATABASE-RESEARCH	GROUP OF CLINICAL PATIENT RESEARCH

Mr Vincent Kindler	Head of group CV	Research subject	Members of the group


	Links about the group Mr Vincent Kindler HUG/Spécialités de Médecine Service d'Hématologie Rue Gabrielle-Perret-Gentil 4 1211 Genève 4 Suisse Vincent.Kindler@hcuge.ch Tel.: +41 22 372 39 42 Comments Pages updated the 17.11.2015		Reseach's subject \| Group's publications \| Research's domains Non leukocyte innate immunity Three main themes are envisaged - Study of MSC differentiation in follicular reticular cells (FRC), pericytes and/or myofibroblasts . FRC are mesenchymatous cells residing in the lymph nodes where they exert a potent regulatory activity on T lymphocytes. A better understanding of their biology and their relationship with MSC would help deciphering how they modulate T cells in situ, keeping in mind that such a control is pivotal for the maintenance of peripheral immune tolerance. - Investigation of the effect of MSC via the activation of indolamine 2,3, deoxygenase (IDO) upon tumor cell growth. IDO, through its ability to metabolize the essential amino acid L-tryptophan modulates many cell types, including T cells and several tumoral cells. The intensity of IDO censoring is related to cell sensitivity to tryptophan depletion. IDO direct effect is expected to inhibit tumor proliferation. However if a tumor becomes IDO-resistant and is surrounded by IDO+ cells, the depletion of tryptophan induced by IDO will inhibit tumor specific T cells and favor tumor growth. A better understanding of these processes should help the development of new aspects of anti-tumoral therapies. - Establishment of a protocol generating clinical grade MSC to be infused to patients suffering from severe GVHD. Many clinical trials suggest that MSC systemic infusion may stop steroid resistant GVHD. However the paucity of GMP grade MSC often prejudices the initiation of such treatment. This project consists in the finalization of the production of MSC in white room conditions. Group's publications Implication of indolamine 2,3 dioxygenase in the tolerance toward fetuses, tumors, and allografts. JOURNAL OF LEUKOCYTE BIOLOGY 2013 vol. 93(5) pp. 681-687 DÜRR S, KINDLER V Autologous bone marrow mononuclear cell transplantation in patients with decompensated alcoholic liver disease: a randomized controlled trial. PLOS ONE 2013 vol. 8(1) pp. 53719-53719 SPAHR L, CHALANDON Y, TERRAZ S, KINDLER V, RUBBIA-BRANDT L, FROSSARD JL, BREQUET R, LANTHIER N, FARINA A, PASSWEG J, BECKER CD, HADENGUE A Impact of selection of cord blood units from the United States and swiss registries on the cost of banking operations. TRANSFUSION MEDICINE AND HEMOTHERAPY 2013 vol. 40(1) pp. 14-20 BART T, BOO M, BALABANOVA S, FISCHER Y, NICOLOSO G, FOEKEN L, OUDSHOORN M, PASSWEG J, TICHELLI A, KINDLER V, KURTZBERG J, PRICE T, REGAN D, SHPALL EJ, SCHWABE R Low molecular weight dextran sulfate binds to human myoblasts and improves their survival after transplantation in mice. CELL TRANSPLANTATION 2012 vol. 0 pp. 0-0 LAUMONIER T, PRADIER A, HOFFMEYER P, KINDLER V, MENETREY J Third-party mesenchymal stromal cell infusion is associated with a decrease in thrombotic microangiopathy symptoms observed post-hematopoietic stem cell transplantation. PEDIATRIC TRANSPLANTATION 2012 vol. 16(2) pp. 131-136 ANSARI M, STRUNK D, SCHALLMOSER K, DELCO C, ROUGEMONT AL, MOLL S, VILLARD J, GUMY PAUSE F, CHALANDON Y, PARVEX P, PASSWEG J, OZSAHIN H, KINDLER V Human bone marrow stromal cells and skin fibroblasts inhibit natural killer cell proliferation and cytotoxic activity. CELL TRANSPLANTATION 2011 vol. 20(5) pp. 681-691 PRADIER A, PASSWEG J, VILLARD J, KINDLER V EWS-FLI-1 expression triggers a Ewing's sarcoma initiation program in primary human mesenchymal stem cells. CANCER RESEARCH 2008 vol. 68(7) pp. 2176-2185 RIGGI N, SUVÀ ML, SUVÀ D, CIRONI L, PROVERO P, TERCIER S, JOSEPH JM, STEHLE JC, BAUMER K, KINDLER V, STAMENKOVIC I In vitro activated human T lymphocytes very efficiently attach to allogenic multipotent mesenchymal stromal cells and transmigrate under them. JOURNAL OF CELLULAR PHYSIOLOGY 2008 vol. 214(3) pp. 588-594 SUVA D, PASSWEG J., ARNAUDEAU S, HOFFMEYER P, KINDLER V Human CD34+ CD11b- cord blood stem cells generate in vitro a CD34- CD11b+ subset that is enriched in langerin+ Langerhans dendritic cell precursors. EXPERIMENTAL HEMATOLOGY 2006 vol. 34(11) pp. 1471-1479 SOULAS C, ARRIGHI JF, SAELAND S, CHAPUIS B, KINDLER V Non-hematopoietic human bone marrow contains long-lasting, pluripotential mesenchymal stem cells JOURNAL OF CELLULAR PHYSIOLOGY 2004 vol. 198 pp. 110-118 SUVA D, GARAVAGLIA G, MENETREY J, CHAPUIS B, HOFFMEYER P, BERNHEIM L, KINDLER V CD34 cord blood cells expressing cutaneous lymphocyte-associated antigen (CLA) are enriched in granulocyte-macrophage progenitors and support an extensive amplification of dendritic cell progenitors EXPERIMENTAL HEMATOLOGY 2001 vol. 29 pp. 1029-1037 ARRIGHI JF, ZUBLER RH, HAUSER C, IRION O, BROWERS N, CHAPUIS B, KINDLER V Transduction of CD34+ cells with lentiviral vectors enables the production of large quantities of transgene-expressing immature and mature dentritic cells THE JOURNAL OF GENE MEDICINE 2001 vol. 3 pp. 311-320 SALMON P, ARRIGHI JF, PIGUET V, CHAPUIS B, ZUBLER RH, TRONO D, KINDLER V Research's domains