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Laboratory of gynecologic tumor and development biology

Placental physio/pathology

The placenta is a temporary organ established to ensure a continuous food and gas supply to the growing foetus. This requires an efficient access of maternal blood to a large transporting epithelial contact area: the syncytiotrophoblast. This is made possible by the rapidly growing cytotrophoblast and ensuing endometrial invasion by the mononuclear cytotrophoblastic cells (CTB). However trophoblast invasion is limited both spatially (to the endometrium and the proximal third of the myometrium) and temporally (to first half of pregnancy). Deregulation of trophoblastic invasiveness and/or differentiation could lead to pregnancy pathologies such as preeclampsia.
Our lab focuses on regulation of trophoblastic cell invasiveness, mechanism of cytotrophoblast differentiation and development of biomarker of preeclampsia.

Role of GRP78 in ovarian carcinogenesis

Glucose-regulated protein 78 (GRP78) is a chaperone protein involved in folding of proteins. It is necessary for the survival of stressed cells such as cancer cells and could induce invasion of tumour cells by an unknown mechanism that we would like to study.
This protein is an endoplasmic reticulum-associated protein but it was also observed on cell membranes on some malignant cells. This localization, specific of some cancer cells, suggests that GRP78 could be a relevant cell surface target for therapeutic intervention. We are interested in the role of GRP78, and particularly membrane GRP78 in ovarian carcinogenesis, and its utility for targeted nanotherapy.

Group's publications

Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy.
MOLECULAR HUMAN REPRODUCTION
2013 vol. 19(1) pp. 43-53
TIRADO-GONZÁLEZ I, FREITAG N, BARRIENTOS G, SHAIKLY V, NAGAEVA O, STRAND M, KJELLBERG L, KLAPP BF, MINCHEVA-NILSSON L, COHEN M, BLOIS SM

Role of prostate apoptosis response 4 in translocation of GRP78 from the endoplasmic reticulum to the cell surface of trophoblastic cells.
PLOS ONE
2013 vol. 8(11) pp. 80231-80231
COHEN M, RIBAUX P, EPINEY M, IRION O

Diagnosis and management of obstetrical antiphospholipid syndrome: where do we stand?
POLISH ARCHIVES OF INTERNAL MEDICINE
2013 vol. 123(12) pp. 713-720
MARCHETTI T, COHEN M, GRIS JC, DE MOERLOOSE P

GRP78-targeted nanotherapy against castrate-resistant prostate cancer cells expressing membrane GRP78.
TARGETED ONCOLOGY
2013 vol. 8(4) pp. 225-230
DELIE F, PETIGNAT P, COHEN M

Anti-KDEL-coated nanoparticles: a promising tumor targeting approach for ovarian cancer?
BIOCHIMIE
2012 vol. 94(11) pp. 2391-2397
DELIE F, RIBAUX P, PETIGNAT P, COHEN M

Overexpression of GRP78 in complete hydatidiform moles.
GYNECOLOGIC ONCOLOGY
2012 vol. 125(3) pp. 580-584
RIBAUX P, PETIGNAT P, FINCI V, NEHMÉ FP, CARLES D, IRION O, EPINEY M, COHEN M

Comparative analysis of secreted proteins from normal and preeclamptic trophoblastic cells using proteomic approaches.
JOURNAL OF PROTEOMICS
2012 vol. 75(6) pp. 1771-1777
EPINEY M, RIBAUX P, ARBOIT P, IRION O, COHEN M

An active product of cruciferous vegetables, 3,3'-diindolylmethane, inhibits invasive properties of extravillous cytotrophoblastic cells.
NEUROENDOCRINOLOGY LETTERS
2012 vol. 33(2) pp. 133-137
RIBAUX P, IRION O, COHEN M

Expression of metalloproteinases 1, 2, 7, 9, and 12 in human cytotrophoblastic cells from normal and preeclamptic placentas.
NEUROENDOCRINOLOGY LETTERS
2012 vol. 33(4) pp. 406-411
COHEN M, RIBAUX P, EPINEY M, IRION O

Involvement of membrane GRP78 in trophoblastic cell fusion.
PLOS ONE
2012 vol. 7(8) pp. 40596-40596
FRADET S, PIERREDON S, RIBAUX P, EPINEY M, SHIN YA K, IRION O, COHEN M

Purified autoantibodies against glucose-regulated protein 78 (GRP78) promote apoptosis and decrease invasiveness of ovarian cancer cells.
CANCER LETTERS
2011 vol. 309(1) pp. 104-109
COHEN M, PETIGNAT P

Heat shock proteins in ovarian cancer: a potential target for therapy.
GYNECOLOGIC ONCOLOGY
2010 vol. 119(1) pp. 164-166
COHEN M, DROMARD M, PETIGNAT P

Alteration of circulating Placental Leucine Aminopeptidase (P-LAP) activity in preeclampsia.
NEUROENDOCRINOLOGY LETTERS
2010 vol. 31(1) pp. 63-66
LANDAU R, LAVERRIERE A, BISCHOF P, IRION O, MORALES M, COHEN M

Role of decidua in trophoblastic invasion.
NEUROENDOCRINOLOGY LETTERS
2010 vol. 31(2) pp. 193-197
COHEN M, WUILLEMIN C, IRION O, BISCHOF P

Immortalized human skin fibroblast feeder cells support growth and maintenance of both human embryonic and induced pluripotent stem cells.
HUMAN REPRODUCTION (OXFORD, ENGLAND)
2009 vol. 24(10) pp. 2567-2581
UNGER C AND AL.

GRP78 as a marker of pre-eclampsia: an exploratory study
MOLECULAR HUMAN REPRODUCTION
2009 vol. 15(9) pp. 569-574
LAVERRIERE A, LANDAU R, CHARVET I, IRION O, BISCHOF P, MORALES M, COHEN M

Glucose-regulated protein 78: a new partner of p53 in trophoblast.
PROTEOMICS
2009 vol. 9(23) pp. 5316-5327
ARNAUDEAU S, ARBOIT P, BISCHOF P, SHIN-YA K, TOMIDA A, TSURUO T, IRION O, COHEN M

Trophoblastic p53 is stabilised by a cis-trans isomerisation necessary for the formation of high molecular weight complexes involving the N-terminus of p53.
BIOCHIMIE
2008 vol. 90(6) pp. 855-862
COHEN M, WUILLEMIN C, BISCHOF P

Regulation of MMP-9 by p53 in first trimester cytotrophoblastic cells.
HUMAN REPRODUCTION (OXFORD, ENGLAND)
2008 vol. 23(10) pp. 2273-2281
COHEN M, WUILLEMIN C, IRION O, BISCHOF P

Effects of ligands or substrate of insulin-regulated aminopeptidase (IRAP) on trophoblast invasion.
JOURNAL OF PEPTIDE SCIENCE : AN OFFICIAL PUBLICATION OF THE EUROPEAN PEPTIDE SOCIETY
2008 vol. 14(7) pp. 883-887
COHEN M, WUILLEMIN C, CHAI SY, BISCHOF P

Factors regulating trophoblast invasion
GYNECOLOGIC AND OBSTETRIC INVESTIGATION
2007 vol. 64(3) pp. 126-130
COHEN M, BISCHOF P

Identification of BARD1 splice-isoforms involved in human trophoblast invasion
INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND CELL BIOLOGY
2007 vol. 39(9) pp. 1659-1672
LI L, COHEN M, WU J, SOW MH, NIKOLIC B, BISCHOF P, IRMINGER-FINGER I

Involvement of MAPK pathway in TNF-alpha-induced MMP-9 expression in human trophoblastic cells
MOLECULAR HUMAN REPRODUCTION
2006 vol. 12(4) pp. 225-232
COHEN M, MEISSER A, HAENGGELI L, BISCHOF P

Metalloproteinases and human placental invasiveness
PLACENTA
2006 vol. 27(8) pp. 783-793
COHEN M, MEISSER A, BISCHOF P

Concentrations of circulating gelatinases (matrix metalloproteinase-2 and -9) are dependent on the conditions of blood collection
CLINICAL CHEMISTRY
2005 vol. 51(1) pp. 274-276
MEISSER ARIELLE, COHEN MARIE-BENOITE, BISCHOF PAUL

Research's domains

REPRODUCTIVE BIOLOGY




DATABASE-RESEARCH	GROUP OF CLINICAL PATIENT RESEARCH

Prof. Marie Cohen	Head of group CV	Research subject	Members of the group


	Links about the group Prof. Marie Cohen Dpt Gynécologie Obstétrique Maternité Boulevard de la Cluse 1211 Genève 14 Suisse Marie.Cohen@hcuge.ch Tel.: +41 22 372 43 81 Comments Pages updated the 17.11.2015		Reseach's subject \| Group's publications \| Research's domains Laboratory of gynecologic tumor and development biology Placental physio/pathology The placenta is a temporary organ established to ensure a continuous food and gas supply to the growing foetus. This requires an efficient access of maternal blood to a large transporting epithelial contact area: the syncytiotrophoblast. This is made possible by the rapidly growing cytotrophoblast and ensuing endometrial invasion by the mononuclear cytotrophoblastic cells (CTB). However trophoblast invasion is limited both spatially (to the endometrium and the proximal third of the myometrium) and temporally (to first half of pregnancy). Deregulation of trophoblastic invasiveness and/or differentiation could lead to pregnancy pathologies such as preeclampsia. Our lab focuses on regulation of trophoblastic cell invasiveness, mechanism of cytotrophoblast differentiation and development of biomarker of preeclampsia. Role of GRP78 in ovarian carcinogenesis Glucose-regulated protein 78 (GRP78) is a chaperone protein involved in folding of proteins. It is necessary for the survival of stressed cells such as cancer cells and could induce invasion of tumour cells by an unknown mechanism that we would like to study. This protein is an endoplasmic reticulum-associated protein but it was also observed on cell membranes on some malignant cells. This localization, specific of some cancer cells, suggests that GRP78 could be a relevant cell surface target for therapeutic intervention. We are interested in the role of GRP78, and particularly membrane GRP78 in ovarian carcinogenesis, and its utility for targeted nanotherapy. Group's publications Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy. MOLECULAR HUMAN REPRODUCTION 2013 vol. 19(1) pp. 43-53 TIRADO-GONZÁLEZ I, FREITAG N, BARRIENTOS G, SHAIKLY V, NAGAEVA O, STRAND M, KJELLBERG L, KLAPP BF, MINCHEVA-NILSSON L, COHEN M, BLOIS SM Role of prostate apoptosis response 4 in translocation of GRP78 from the endoplasmic reticulum to the cell surface of trophoblastic cells. PLOS ONE 2013 vol. 8(11) pp. 80231-80231 COHEN M, RIBAUX P, EPINEY M, IRION O Diagnosis and management of obstetrical antiphospholipid syndrome: where do we stand? POLISH ARCHIVES OF INTERNAL MEDICINE 2013 vol. 123(12) pp. 713-720 MARCHETTI T, COHEN M, GRIS JC, DE MOERLOOSE P GRP78-targeted nanotherapy against castrate-resistant prostate cancer cells expressing membrane GRP78. TARGETED ONCOLOGY 2013 vol. 8(4) pp. 225-230 DELIE F, PETIGNAT P, COHEN M Anti-KDEL-coated nanoparticles: a promising tumor targeting approach for ovarian cancer? BIOCHIMIE 2012 vol. 94(11) pp. 2391-2397 DELIE F, RIBAUX P, PETIGNAT P, COHEN M Overexpression of GRP78 in complete hydatidiform moles. GYNECOLOGIC ONCOLOGY 2012 vol. 125(3) pp. 580-584 RIBAUX P, PETIGNAT P, FINCI V, NEHMÉ FP, CARLES D, IRION O, EPINEY M, COHEN M Comparative analysis of secreted proteins from normal and preeclamptic trophoblastic cells using proteomic approaches. JOURNAL OF PROTEOMICS 2012 vol. 75(6) pp. 1771-1777 EPINEY M, RIBAUX P, ARBOIT P, IRION O, COHEN M An active product of cruciferous vegetables, 3,3'-diindolylmethane, inhibits invasive properties of extravillous cytotrophoblastic cells. NEUROENDOCRINOLOGY LETTERS 2012 vol. 33(2) pp. 133-137 RIBAUX P, IRION O, COHEN M Expression of metalloproteinases 1, 2, 7, 9, and 12 in human cytotrophoblastic cells from normal and preeclamptic placentas. NEUROENDOCRINOLOGY LETTERS 2012 vol. 33(4) pp. 406-411 COHEN M, RIBAUX P, EPINEY M, IRION O Involvement of membrane GRP78 in trophoblastic cell fusion. PLOS ONE 2012 vol. 7(8) pp. 40596-40596 FRADET S, PIERREDON S, RIBAUX P, EPINEY M, SHIN YA K, IRION O, COHEN M Purified autoantibodies against glucose-regulated protein 78 (GRP78) promote apoptosis and decrease invasiveness of ovarian cancer cells. CANCER LETTERS 2011 vol. 309(1) pp. 104-109 COHEN M, PETIGNAT P Heat shock proteins in ovarian cancer: a potential target for therapy. GYNECOLOGIC ONCOLOGY 2010 vol. 119(1) pp. 164-166 COHEN M, DROMARD M, PETIGNAT P Alteration of circulating Placental Leucine Aminopeptidase (P-LAP) activity in preeclampsia. NEUROENDOCRINOLOGY LETTERS 2010 vol. 31(1) pp. 63-66 LANDAU R, LAVERRIERE A, BISCHOF P, IRION O, MORALES M, COHEN M Role of decidua in trophoblastic invasion. NEUROENDOCRINOLOGY LETTERS 2010 vol. 31(2) pp. 193-197 COHEN M, WUILLEMIN C, IRION O, BISCHOF P Immortalized human skin fibroblast feeder cells support growth and maintenance of both human embryonic and induced pluripotent stem cells. HUMAN REPRODUCTION (OXFORD, ENGLAND) 2009 vol. 24(10) pp. 2567-2581 UNGER C AND AL. GRP78 as a marker of pre-eclampsia: an exploratory study MOLECULAR HUMAN REPRODUCTION 2009 vol. 15(9) pp. 569-574 LAVERRIERE A, LANDAU R, CHARVET I, IRION O, BISCHOF P, MORALES M, COHEN M Glucose-regulated protein 78: a new partner of p53 in trophoblast. PROTEOMICS 2009 vol. 9(23) pp. 5316-5327 ARNAUDEAU S, ARBOIT P, BISCHOF P, SHIN-YA K, TOMIDA A, TSURUO T, IRION O, COHEN M Trophoblastic p53 is stabilised by a cis-trans isomerisation necessary for the formation of high molecular weight complexes involving the N-terminus of p53. BIOCHIMIE 2008 vol. 90(6) pp. 855-862 COHEN M, WUILLEMIN C, BISCHOF P Regulation of MMP-9 by p53 in first trimester cytotrophoblastic cells. HUMAN REPRODUCTION (OXFORD, ENGLAND) 2008 vol. 23(10) pp. 2273-2281 COHEN M, WUILLEMIN C, IRION O, BISCHOF P Effects of ligands or substrate of insulin-regulated aminopeptidase (IRAP) on trophoblast invasion. JOURNAL OF PEPTIDE SCIENCE : AN OFFICIAL PUBLICATION OF THE EUROPEAN PEPTIDE SOCIETY 2008 vol. 14(7) pp. 883-887 COHEN M, WUILLEMIN C, CHAI SY, BISCHOF P Factors regulating trophoblast invasion GYNECOLOGIC AND OBSTETRIC INVESTIGATION 2007 vol. 64(3) pp. 126-130 COHEN M, BISCHOF P Identification of BARD1 splice-isoforms involved in human trophoblast invasion INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND CELL BIOLOGY 2007 vol. 39(9) pp. 1659-1672 LI L, COHEN M, WU J, SOW MH, NIKOLIC B, BISCHOF P, IRMINGER-FINGER I Involvement of MAPK pathway in TNF-alpha-induced MMP-9 expression in human trophoblastic cells MOLECULAR HUMAN REPRODUCTION 2006 vol. 12(4) pp. 225-232 COHEN M, MEISSER A, HAENGGELI L, BISCHOF P Metalloproteinases and human placental invasiveness PLACENTA 2006 vol. 27(8) pp. 783-793 COHEN M, MEISSER A, BISCHOF P Concentrations of circulating gelatinases (matrix metalloproteinase-2 and -9) are dependent on the conditions of blood collection CLINICAL CHEMISTRY 2005 vol. 51(1) pp. 274-276 MEISSER ARIELLE, COHEN MARIE-BENOITE, BISCHOF PAUL Research's domains REPRODUCTIVE BIOLOGY